We are seeking an experienced neuroscientist who wishes to contribute to our research programme investigating the genetic basis of circadian rhythms, sleep and behavior in mice. Our research involves generating and characterizing mouse mutant lines and our investigations have led to new and important insights into how mutations in genes affect these behaviours (see Parsons et al, Cell (2015) 162: 607-21; Wilcox et al, J Biol Rhythms (2017) 32: 433-443). We are based at MRC Harwell, a major international research centre at the forefront of studies in mouse genetics and functional genomics. The centre investigates a wide variety of mouse models in order to enhance our understanding of the genetic basis of physiology and disease.
As a neurogeneticist, your key responsibility will be the in depth characterization of the mutant lines we have generated. This will include the use of tools and approaches to investigate gene function using mouse genetic models. Our current interests are focused on the region-specific manipulation of gene expression using stereotaxic administration of viral vectors (eg. AAV). Experience in the application of these approaches to neuroscience research would be highly desirable. Experience in the use of in vivo telemetric recording would also be advantageous.
You will work as part of a team investigating multidisciplinary aspects of mouse neurobiology with a focus on biological rhythms and sleep. The team includes a programme leader, junior and senior postdoctoral researchers and DPhil students.
To join us, you will need:
• A track record in an appropriate discipline (e.g. genetics,
• Experience in whole animal and/or ex vivo techniques
• Experience with use of viral vectors (eg. AAV)
• The ability to work in a multi-disciplinary team and undertake
• Excellent communication and organisation skills and a flexible, pro-
Education / Qualifications / Training required:
Experience in in vivo neuroscience tools including stereotaxic surgery, use of AAV vectors
PhD in appropriate discipline (e.g. Genetics, Neuroscience)
Knowledge and experience:
Proven track record with publications
Mouse colony management, personal licence holder
Criteria 1: Molecular Genetics
Subcloning, use of expression vectors, use of viral vectors
Viral manipulation of gene function in cells, ex vivo or whole animal
Criteria 2: Mouse behaviour
Experience in mouse behaviour with focus on circadian rhythms
Mouse EEG telemetry experience, electrophysiology
Criteria 3: Mouse neurophysiology
Brain ex vivo work, immunofluorescence
Criteria 4: Communication/Team
Ability to document and organise work
Ability to present clearly
Ability to work as a team
Criteria 5: Methodology
Ability to plan work appropriately, data analysis
Ability to independently work to problem description and requirements
Criteria 6: Computer Skills General
Word, Excel, Powerpoint , Statistical analysis
Personal style and behaviour
Personable, imaginative, hardworking, team player.
Security clearance will be required for the successful candidate.