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Proteomics Separations - Postdoctoral Research Associate

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The Rosalind Franklin Institute (the Franklin) is a new national Research Centre, funded by the UK government through UK Research and Innovation, dedicated to bringing transformative changes in life science through interdisciplinary research and technology development.

We are currently expanding and looking to recruit a Postdoctoral Research Associate as part of our Next Generation Chemistry theme, specifically to work within the proteomics team. One of the major goals of all themes is to improve their technologies by a factor of ten. In the case of this role, we want to improve the sensitivity of cutting-edge experiments trying to reach single cell proteomics. It is little appreciated that the rate and power of MS analysis is outstripping the key chemical techniques that are coupled to it. One of the understated and most powerful aspects of the proteomics experiment is the peptide/protein chromatographic separation, new modes in chromatography have the power unleash the power of MS in a way that will unlock a new dimension of Biology hitherto unseen.

This role is dedicated to creating new ultra-high-performance separations for proteomics that will be applied to a range of pertinent biochemical and clinical systems in collaboration with the other themes and research groups in the stakeholder universities.

The successful candidate will create, develop, and characterise several peptide separation technologies. One key developmental aspect will be to create ultra-narrow columns to maximise sensitivity. These ultra-low flow nanoLC systems will be coupled to several state-of-the-art mass spectrometers for cutting edge proteomics experiments. When sufficiently robust, the system will be utilised for a range of biochemical and clinical systems in collaboration with other themes and research groups in the stakeholder universities.

The project is a collaboration with other themes at the Franklin and with industrial (Evosep) and academic collaborators, with whom you will work extensively, while liaising with academic experts in the UK and beyond. The post, which is for 3 years in the first instance, reports to the Franklin’s Head of Mechanistic Proteomics, and will work alongside other researchers in the Franklin. Please email informal enquiries to Shabaz.Mohammed@chem.ox.ac.uk.

Role overview and responsibilities

We are currently looking for an experienced Proteomics Separations - Postdoctoral Research Associate with excellent skills in the following areas:

• Practical experience with nanolitre flow rate LC systems.

• Familiarity with a wide range of chromatographies employed in proteomics

• Practical experience with hyphenating LCs to mass spectrometers.

• The ability to carry out analytical procedures that are applied for proteome characterisation to a high degree of precision.

• Able to analyse quantitative proteomic data with a degree of statistical rigour using common freely available software packages.

The main specific responsibilities of this role will include:

• Create, develop, and characterise a range of nanolitre flow rate LC systems.

• Hyphenate LCs to a range of state-of-the-art mass spectrometers.

• Carry out analytical procedures that are applied for proteome characterisation to a high degree of precision including sample preparation.

• Analyse quantitative proteomic data with a degree of statistical rigour using common freely available software packages.

• Act as a source of information and advice to other members of the group on scientific protocols and experimental techniques

• Assist with general laboratory maintenance including the troubleshooting and upkeep of nanoLCs, centrifugal evaporators, liquid handlers and various MS equipment.

You will also

• Collaborate with appropriate academic and industrial partners.

• Work with other themes and researchers to develop the burgeoning Franklin environment to be as suitable as possible for such work.

• Work together with the surrounding facilities to ensure efficient methods at the Franklin and Harwell environment.

• Publish research articles in leading academic journals.

• Present findings at national and international meetings/conferences.

• Take a formal or informal role (as required) in the supervision of research students, including recruitment, mentorship and career development.

• Raise research funds through grant applications, and manage own area of a larger research budget

• Participate in and support the public engagement and widening access activities of the RFI

• Any other duties that come up in the normal running of the facility and that can be reasonably requested of the post-holder.

Selection criteria

Essential

• A PhD (or close to completion) or equivalent in a relevant field.

• Practical experience with chromatographic separations.

• Practical experience with mass spectrometers.

• Practical experience with software packages utilised in mass spectrometry-based proteomics.

• Ability to co-ordinate multiple aspects of work to meet deadlines.

• Ability to work as part of a team as well as independently.

• Ability to solve problems using innovative and flexible thinking.

• Excellent organisational and communication skills.

• Experience with developing routinely usable methods.

• Experience with devising research questions and to develop strategies to address them.

• Ability to travel occasionally for training or dissemination of your work.

• Experience with project management and demonstrated ability to deliver agreed milestones in a timely manner, adapt to changing priorities.

• Ability to discuss and coordinate experiments with a variety of colleagues from different disciplines.

Desirable

• Practical experience of developing and constructing nanoLC systems including column packing.

• Practical experience with proteomics sample preparation.

• Proficiency in handling large datasets including bioinformatics and biostatistics.

• Knowledge of complementary structural and biophysical methods.

• Knowledge of biological chemistry.

In return we offer:

• 25 days holidays

• Generous pension scheme (employer’s contribution currently up to 18%)

• Training and development opportunities for staff at all levels

• Bus pass discount scheme

• Access to employee discount platform

• Subsidised canteen

• Free on-site parking

• Campus location in beautiful countryside with social and sports clubs open to staff

Closing date: 1st February 2021 at 9:00am

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